890 Development of pigmented reconstructed human epidermis model containing human melanoblasts from keratinocyte culture
نویسندگان
چکیده
منابع مشابه
Human Keratinocyte Culture
Stratification of human epidermal cells into multilayered sheets composed of basal and suprabasal layers (resembling the stratum germinativum and stratum spinosum of the epidermis) was studied in a dermal component-free culture system. Although no stratum corneum developed in vitro, this culture system provided a method to study early events in human keratinocyte differentiation. Multiparameter...
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RHE has the ultimate advantage to grow donor epidermal cells in a serumfree culture environment: after rapidly proliferating preparative keratinocyte cultures have been obtained, the yielded epidermal cells are seeded on inert filter substrates that are lifted to the air-liquid interface in a humidified-air incubator. A fully-defined nutrient medium feeds the basal cells through the filter subs...
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Purpose: To compare effects of polarized epithelial monolayer prepared from human oviduct and uterus on mouse one and two-cell embryo development. Materials and Methods: Human oviduct and endometrial tissue was obtained from patients who had undergone total hysterectomy. The epithelial cells were isolated from tissue and cultured on extracellular matrix (ECM) Gel coated Millipore filter insert...
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CONTEXT AND OBJECTIVE The technique of obtaining human skin with dermis and epidermis reconstructed from cells isolated from patients can enable autologous skin grafting on patients with few donor sites. It also enables in vitro trials on chemicals and drugs. The objective of this work was to demonstrate a method for obtaining human skin composed of associated dermis and epidermis, reconstructe...
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BACKGROUND Trichophyton rubrum represents the most common infectious fungus responsible for dermatophytosis in human, but the mechanism involved is still not completely understood. An appropriate model constructed to simulate host infection is the prerequisite to study the pathogenesis of dermatophytosis caused by T. rubrum. In this study, we intended to develop a new T. rubrum infection model ...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2019
ISSN: 0022-202X
DOI: 10.1016/j.jid.2019.03.966